0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Yeast
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
Application Notes:
Rub1 Antibody is tested in western blot and defects a 6 kDa band corresponding to yeast Rub1. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Anti-Rub1 antibody, generated by immunization with full-length, recombinant yeast Rub1, was tested by western blot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4 C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code 611-703-127) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
* VAT and and shipping costs not included. Errors and price changes excepted
