This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast SUMO protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-SUMO Antibody, Ubiquitin-like protein SMT3 antibody, SMT3 antibody, Ubiquitin like protein of the SUMO family antibody, SMT3_YEAST antibody, DmSUMO 1 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Yeast
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:25,000, ChIP: User Optimized, IP: User Optimized, WB: 1:500 - 1:3,000
Application Notes:
Anti-Sumo purified polyclonal antibody reacts yeast SUMO tested by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1,000 dilution is recommended. A 12
Western blot of SUMO-GFP fusion proteins cleaved by insect cell protein extracts. Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 µg E. coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 µg total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with Anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60C and were then re-probed using the Anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey Anti-Rabbit IgG (code 611-703-127) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
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