SKG-1 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: SKG-1 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-401-844
• Supplier Catalog Number: 200-401-844
• Alternative Catalog Number: ROC-200-401-844
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human
• Immunogen: SKG-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 400-431 of human SGK-1.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-SKG-1 Antibody, Serum/glucocorticoid-regulated kinase 1, SGK 1 antibody, SGK1 antibody, Cellular stress response antibodies, Serine/threonine-protein kinase Sgk1

Product Properties

• Clonality: Polyclonal
• Concentration: 5.0 mg/mL by UV absorbance at 280 nm
• NCBI: 6446
• UniProt: O00141
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:4,000 - 1:16,000, IHC: 10 µg/ml to 20 µg/ml, WB: 1:500 - 1:3,000
• Application Notes: SKG-1 Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~49 kDa in size corresponding to SGK-1 by western blotting in the appropriate

Images

Rocklands Affinity Purified anti-SGK-1 antibody was used at a 15 µg/ml to detect nuclear and cytoplasmic signal in a variety of tissues including adrenal, heart, liver, ovary, pancreas, placenta, skin, spleen, testes, thyroid and uterus. Low to moderate levels of background staining were noted. This image shows SGK-1 staining of human pancreas. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.