Anti-RREB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to mouse RREB1 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-RREB1 Antibody, rabbit anti-RREB 1 Antibody, FINB antibody, Finger protein in nuclear bodies antibody, LZ321 antibody, Raf responsive zinc finger protein LZ321 antibody, RAS responsive element binding protein 1 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2,000 - 1:15,000, WB: 1:500 - 1:2,000
Application Notes:
This Protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 90-180 kDa in size corresponding to RREB1 by western bl
Western blot using Rocklands Protein A Purified anti-RREB1 antibody shows detection of a predominant band believed to be RREB1 in various cell lysates (1 - HEK293, 2 - RFP-RREB transfected HEK293, 3 - M460 and 4 - T1165). All lysates were loaded at 20 µg per lane and separated by SDS-PAGE. After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1,000. The membrane was washed and reacted with IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication, Shuling Zhang, CCR, NCI, Bethesda, MD.
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