Anti-SUMO Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:20,000, IF Microscopy: User Optimized, WB: 1:500 - 1:2,000
Application Notes:
This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~60 kDa in size corresponding to SAE1 by western blotting in the appropriate cell lysate or
(Panel A) Coomassie-stained SDS-PAGE and (Panel B) western blotting of anti-SUMO Activating Enzyme (SAE1) antibody. (Panel A) Lane 1: MW Markers. Lane 2: GST-SAE1 recombinant protein [50ng]. (Panel B) Lane 1: MW Markers ]red, 700 nm channel]. Lane 2: HeLa WC lysate [35µg]. Lane 3: purified recombinant GST-SAE1 [50ng]. Lane 4: purified GST [300ng]. Primary Antibody: anti-SUMO Activating Enzyme (SAE1) at 1:2000 overnight at 4C. Secondary Antibody: 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX Hu (611-132-122) for 45 min at room temperature. Results: The recombinant protein (with tag) ~60 kDa band present [green, 800nm channel]. IRDye(TM)800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. SDS-PAGE image courtesy of Proteome Resources, Englewood, CO, http://www.proteomeresources.com.
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