Selenophosphate Synthetase 2 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Selenophosphate Synthetase 2 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-401-999
• Supplier Catalog Number: 200-401-999
• Alternative Catalog Number: ROC-200-401-999
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Mouse
• Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS2.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Selenophosphate Synthetase 2 Antibody, rabbit anti-SPS 2 antibody, Selenide water dikinase 2 antibody, Selenium donor protein 2 antibody, SEPHS2 antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 2.3 mg/mL by UV absorbance at 280 nm
• NCBI: 20768
• UniProt: P97364
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000
• Application Notes: This Protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to SPS2 by western blotting in the ap

Images

Western blot using Rocklands Protein A purified anti-SPS2 antibody shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.