ASPP2 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-200-401-A19
Article Name: ASPP2 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-200-401-A19
Supplier Catalog Number: 200-401-A19
Alternative Catalog Number: ROC-200-401-A19
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP2.
Conjugation: Unconjugated
Alternative Names: rabbit anti-ASPP2 antibody, ASPP-2, ASPP 2, Apoptosis stimulating of p53 protein 2 antibody, Bbp antibody, Bcl2 binding protein antibody, NY REN 51 antigen antibody, p53 binding protein 2 antibody, Tumor suppressor p53-binding protein 2, TP53BP2
Clonality: Polyclonal
Concentration: 2.4 mg/mL by UV absorbance at 280 nm
NCBI: 7159
UniProt: Q13625
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:2,500 - 1:10,000, IHC: User Optimized, WB: 1:500 - 1:3,000
Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kDa in size corresponding to ASPP2 by western blotting in the a
Western blot using Rocklands Protein A purified anti-ASPP2 to detect over-expressed ASPP2 in MCF-7 cells (Lane 2, arrowhead). Lane 1 is a nontransfected control. Lane 3 is MCF-7 cells over-expressing ASPP1. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 40kDa is unknown. Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.