This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP8 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-FKBP8 Antibody, FKBP 8, FKBP-38, 38 kDa FK 506 binding protein homolog antibody, 38kDa antibody, FK506 binding protein 8 antibody, FKBP 38 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:25,000, WB: 1:500-1:2,000
Application Notes:
This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 64 kDa in size corresponding to FKBP8 by western blotting in the a
Western blot using Rocklands protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 µg of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4 C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.
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