This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of mouse toll-like receptor 11 (TLR11) protein.
This IgG fraction antibody has been tested for use in ELISA, immunocytochemistry, immunofluorescence, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~90 kDa in size corresponding to TLR11 by
Western Blot of Rabbit Anti-TLR11 Antibody. Lane 1: RAW264.7 whole cell lysate using 0.5 µg/ml. Lane 2: RAW264.7 whole cell lysate using 1.0 µg/ml. Load: 10 µg per lane. Primary antibody: TLR-11 antibody overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 106 kDa, ~90 kDa for TLR11. Other band(s): none.
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