TLR1 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-200-401-A68
Article Name: TLR1 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-200-401-A68
Supplier Catalog Number: 200-401-A68
Alternative Catalog Number: ROC-200-401-A68
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human toll-like receptor 1 (TLR1) protein.
Conjugation: Unconjugated
Alternative Names: CD281 antigen antibody, rsc786 antibody, Similar to Drosophila Toll protein antibody, TIL antibody, Toll (Drosophila) homolog antibody, Toll like receptor 1 Precursor antibody
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 7096
UniProt: Q15399
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:10,000 - 1:40,000, IHC: 10 µg/ml, IF Microscopy: 20 µg/mL, WB: 1 - 4 µg/ml
Application Notes: This IgG fraction antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~90 kDa in size corresponding to TLR1 by
Western Blot of Rabbit Anti-TLR1 Antibody. Lane 1: mouse spleen tissue whole cell lysate using 1.0 ug/ml. Lane 2: mouse spleen tissue whole cell lysate using 2.0 ug/ml. Lane 3: mouse spleen tissue whole cell lysate using 4.0 ug/ml. Load: 10 µg per lane. Primary antibody: TLR-1 antibody overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 90 kDa,~90 kDa for TLR 1. Other band(s): none.