Anti-Caspase-10 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid peptide near the C-terminus of human Caspase-10.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1 µg/mL
Application Notes:
Anti-Caspase-10 Antibody has been tested for use in ELISA, immunocytochemistry, immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 59 kDa in Western Blots of sp
Western Blot of Rabbit anti-Caspase-10 antibody. Lane 1: HeLa whole cell lysate (H). Lane 2: Jurkat whole cell lysate (J). Lane 3: A431 whole cell lysate (A). Lane 4: K562 whole cell lysate (K). Load: 35 µg per lane. Primary antibody: anti-Caspase-10 antibody at 1 µg/mL for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 59 kDa, 59 kDa for Caspase-10. Other band(s): None.
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