Anti-DR4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 20 amino acid peptide near the N-terminus of human DR4.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000, IF Microscopy: 5 µg/mL, WB: 1:5,000
Application Notes:
Anti-DR4 Antibody has been tested for use in ELISA, immunocytochemistry, immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 50 kDa in Western Blots of specific
Western Blot of Rabbit anti-DR4 antibody. Lane 1: HeLa whole cell lysate (H). Lane2: K562 whole cell lysate (K). Lane 3: Jurat whole cell lysate (J). Load: 35 µg per lane. Primary antibody: anti-DR4 antibody at 1:500 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 50 kDa, 50 kDa for DR4. Other band(s): None.
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