This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-IL21 Antibody, Interleukin-21, IL-21, Interleukin21, Interleukin 21, IL 21
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Bovine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:6,000, WB: 1 µg/mL
Application Notes:
IL-21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes, and in HL-60 and THP-1 cell lines. This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions fo
Western blot using Rocklands anti-bovine IL-21 antibody shows detection of recombinant bovine IL-21 at 15.1kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rocklands, Inc. Anti-bovine IL-21. After washing, membrane was probed with Dylight (TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody (611-743-127).
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