This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 70 amino acids of the mature swine CCL3L1 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Swine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: User Optimized, WB: 1 µg/ml
Application Notes:
This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kDa in size corresponding to swine CCL3L1 protein by western b
Western blot using Rocklands protein-A purified anti-swine CCL3L1 antibody shows detection of recombinant swine CCL3L1 at 7.8kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rocklands, Inc. Anti-swine CCL3L1. After washing, membrane was probed with Dylight (TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody (611-743-127).
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