This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 164 amino acids of the mature bovine VEGF-A protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-VEGF-A Antibody, Vascular endothelial growth factor A, VEGF A, VEGFA, Vascular permeability factor, VPF
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Bovine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: User Optimized, WB: 1:333
Application Notes:
This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.2 kDa in size corresponding to bovine VEGF-A protein by western
Western blot using Rocklands Protein-A Purified anti-bovine VEGF-A antibody shows detection of recombinant bovine VEGF-A at 17-19.2 kDa. Approximately 2 µg of recombinant protein was loaded per lane onto a 4-20% gradient gel followed by transfer to PVDF membrane. The membrane was blocked using 3% BSA (p/n BSA-30) diluted 1:10. The primary antibody was used at a 1:333 dilution and was incubated with the blot for 2h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(TM)800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers. Other detection systems will yield similar results.
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