This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Tobacco Etch Virus
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:1,500-1:27,000, WB: 1:500
Application Notes:
This protein-A purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate c
Ecoli lysate containing recombinant TEV protease was loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 1% BSA (p/n BSA-30) in TBS for 30min at ambient. The membrane was probed with the primary antibody at a 1:1,000 dilution in 1%BSA/TBS overnight at 4 C. For detection HRP conjugated Gt-a-Rabbit IgG (p/n 611-103-122) was used at a 1:40,000 dilution for 30 min at ambient and data generated with FemtoMax(TM) enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using BioRad Versadoc 4000MP Imaging System. Expect a band approximately 27 kDa.
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