0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Borrelia burgdorferi
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2,000, WB: 1:1,000
Application Notes:
This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 25.4 kDa in size corresponding to Borrelia burgdorferi CRASP-2 protein
Western Blot showing detection of 0.1 µg of recombinant CRASP-2 protein. Lane 1: Molecular weight markers. Lane 2: MBP-CRASP-2 fusion protein (arrow, expected MW = 67.8 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc(TM) MP 4000 imaging system (Bio-Rad).
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