Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 5.0 ug/ml, IHC: User Optimized, WB: 1.0 ug/ml
Application Notes:
Histone H3 antibody has been tested for use in ELISA and western blot. This antibody is suitable for IHC. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should
Western Blot of Rabbit Anti-Histone H3 antibody. Lane 1: Hela WCL (p/n W09-000-364). Lane 2: Hela Nuclear Extract (p/n W09-001-367). Load: 10 µg per lane. Primary antibody: Histone H3 antibody at 1.0 µg/ml for 1 hr at RT. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: ~15 kDa, ~15 kDa for Histone H3.
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