Amyloid Fibrils (OC) Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Amyloid Fibrils (OC) Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-401-E87
• Supplier Catalog Number: 200-401-E87
• Alternative Catalog Number: ROC-200-401-E87
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human
• Immunogen: Amyloid Fibrils (OC) Antibody was produced from whole rabbit serum prepared by repeated immunizations with fibrils prepared from human Abeta42 synthetic peptide.
• Conjugation: Unconjugated
• Alternative Names: Amyloid OC, Fibrils, Amyloid Oligomer alphabeta, A11, Amyloid beta A4 protein, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, APP, A4, AD1

Product Properties

• Clonality: Polyclonal
• NCBI: 05067
• UniProt: P05067
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:200, IHC: User Optimized, IP: User Optimized, WB: 1:1000
• Application Notes: Anti-Amyloid Fibrils (OC) Antibody is tested for use in IP, IF microscopy, IHC, and WB. Specific conditions for reactivity should be optimized by the end user.

Images

Immunohistochemistry of Rabbit anti-Amyloid Fibrils antibody. Tissue: (A) hippocampus, (B) subiculum, (C) temporal cortex, and (D) dense and fine fibrillar material. Fixation: N/A. Primary Antibody: Amyloid Fibrils antibody at 1ug/ml for 1h at RT. Secondary antibody: Peroxidase rabbit secondary at 1:10,000 for 45 min at RT. Localization: Membrane. Staining: Amyloid Fibrils as precipitated brown signal with hematoxylin purple nuclear counterstain.

Dot Blot of Rabbit anti-Amyloid Fibrils antibody. Antigen: Beta Amyloid HEPES-NaCl aggregation. Primary antibody: Amyloid Fibrils antibody at 1:500 (lane 1) and 1:5000 (lane 2) for 45 min at 4C. Secondary antibody: Peroxidase rabbit Secondary antibody at 1:10,000 time lapse. Block: 5% BLOTTO overnight at 4C.

Dot Blot of Rabbit Amyloid Fibrils (OC) antibody. Antigen: Abeta42 and polyQ36 prefibrillar oligomers and fibrils. Load: 2ug per dot. Primary antibody: Top row: Amyloid Oligomers (A11) or bottom row: Amyloid Fibrils (OC) at 1:400 for 45 min at 4C. Secondary Antibody: Goat anti-rabbit IgG HRP at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight at 4C. Amyloid Fibrils (OC) reacts to Abeta42 fibrils and polyQ36 fibrils only.

Western Blot of rabbit Anti-Amyloid Fibrils Antibody. Lane 1 and 3: (F) Fibrils. Lane 2 and 4: (O) prefibrillar oligomers. Load: 10 ug per lane. Primary antibody: Anti-Amyloid Fibrils or Anti-Oligomers at 1:1000 for overnight at 4C . Secondary antibody: Goat anti-rabbit IgG HRP antibody at 1:40,000 for 45 min at RT. Block: 5% Blotto overnight at 4C. Predicted/Observed size: 18kDa on left blot (OC) in lane one.

Immunohistochemistry of Rabbit anti-Amyloid Fibrils antibody. Tissue: Human AD Brain. Fixation: N/A. Primary Antibody: (Left) Amyloid Fibril antibody, (Right) Monoclonal 6E10 at 1ug/ml for 1h at RT. Secondary antibody: Peroxidase rabbit secondary at 1:10,000 for 45 min at RT. Localization: Membrane. Staining: (Left) Amyloid Fibrils as precipiated brown signal with no cross reactivity with Amyloid Precursor Protein (APP), (right) considerable cross reactivity.