HSP60 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: HSP60 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-401-F62
• Supplier Catalog Number: 200-401-F62
• Alternative Catalog Number: ROC-200-401-F62
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Bovine, Canine, Gallus, Human, Mouse, Rabbit, Rat
• Immunogen: Hsp60 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against human Hsp60 produced through recombinant DNA methods in E.coli.
• Conjugation: Unconjugated
• Alternative Names: CPN60, GROEL, HLD4, Hsp 60, Hsp65, HSPD1, HuCHA60, SPG 13, 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, Mitochondrial matrix protein P1, P60 lymphocyte protein, PD1

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml by UV absorbance at 280 nm
• NCBI: 3329
• UniProt: P10809
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:200, IHC: User Optimized, IP: User Optimized, WB: 1:1000
• Application Notes: Anti-HSP60 Antibody is tested for use in WB, IHC, IF and IP. Expect a band approximately ~60kDa protein corresponding to the molecular mass of Hsp60 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of rabbit anti-Hsp60 antibody. Lane 1: 100ng Hsp60 recombinant protein. Lane 2: 7.5µg SKBR3 human lysate. Lane 3: 7.5µg MDCK dog lysate. Lane 4: 7.5µg MEF mouse lysate. Primary antibody: Hsp60 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 61.1 kDa for Hsp60. Other band(s): none.