Anti-GAB1 Antibody was produced in rabbits by repeated immunizations with a recombinant protein produced in Sf9 cells corresponding to the full length human GAB protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit Anti-GAB1 Antibody, GAB 1, GAB-1, GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2,000 - 1:10,000, WB: 1:500-1:2000
Application Notes:
Anti-GAB1 Antibody has been tested by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at 76.6 kDa by western blotting in the appropriate cell lysate or extract.
Western Blot of Rabbit anti-GAB1 antibody. Lane 1: MCF7 lysate (p/n W09-000-360). Lane 2: GAB1 rProtein (p/n 009-001-G66). Lane 3: GST protein (p/n 000-001-200). Load: 0.05 µg of protein or 30µg of lysate. Primary antibody: GAB1 antibody at 1µg/mL for overnight at 4C. Secondary antibody: Goat-anti-Rabbit IgG HRP antibody (p/n 611-103-122) at 1:40,000 for 45 min at RT. Block: MB-070 Fluorescent blocking buffer overnight at 4C. Predicted/Observed size: 130 kDa for GAB-1. Other band(s): none.
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