Gremlin Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the internal amino acid sequences within the Gremlin-1 human protein.
Conjugation:
Unconjugated
Alternative Names:
CKTSF1B1, DAND2, DRM, Gremlin-1, Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, BMP antagonist 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, Increased in high glucose protein 2
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
IHC: 2.5-10 µg/mL (paraffin), WB: 1:500-1:1000
Application Notes:
Anti-Gremlin Antibody is tested for use in IHC (cryosections) and IHC-P. Specific conditions for reactivity should be optimized by the end user.
Immunohistochemistry of Rabbit Anti-Gremlin antibody. Tissue: human colon cancer. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Gremlin antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Gremlin is stained brown.
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