0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
WB: 1-3 µg/mL
Application Notes:
Anti-NFATc1 Antibody is tested for WB and ICC/IF. Expect a band approximately 90-110kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit Anti-NFATc1 antibody. Lane 1: human Ramos. Lane 2: mouse RAW cell lysate. Primary antibody: NFATc1 at 1µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~80 kDa for NFATc1. Other band(s): none.
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