SIRT5 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to internal amino acids of human SIRT5 .
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
WB: 1-3 µg/mL
Application Notes:
Anti-SIRT5 Antibody is tested for use in WB. Expect a band approximately 37kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit Anti-SIRT5 antibody. Lane A: human intestine. Lane B: mouse intestine. Lane C: rat intestine cell lysate. Primary antibody: SIRT5 at 2 µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~34 kDa for SIRT5. Other band(s): none.
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