SMAD6 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to two internal amino acid sequences in the human SMAD6 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
IHC: 5-10 µg/mL, WB: 1-3 µg/mL
Application Notes:
Anti-SMAD6 antibody is tested for use in IHC, IF, and WB. Expect a band approximately 53kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit Anti-SMAD6 antibody. Lane A: Lysate from Jurkat cells. Primary antibody: SMAD6 at 2 µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for SMAD6. Other band(s): none.
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