TLR5 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: TLR5 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-401-I28
• Supplier Catalog Number: 200-401-I28
• Alternative Catalog Number: ROC-200-401-I28
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: FC, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: TLR5 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a KLH-conjugated peptide corresponding to internal amino acids on human TLR5 protein.
• Conjugation: Unconjugated
• Alternative Names: TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 7100
• UniProt: O60602
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 1-2 µg/10 6 cells, IHC: 10 µg/mL (paraffin), WB: 1-3 µg/mL
• Application Notes: Anti-TLR5 Antibody is tested for use in WB, Flow, Flow-CS, IHC, and IHC-P. Expect a band approximately 97kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Images

Flow Cytometry of Rabbit Anti-TLR5 antibody. Cells: Balb/c mouse splenocytes. Stimulation: none. Primary Antibody: TLR5 antibody at 2 ug/10 6 cells (red) and rabbit IgG isotype control (green). Secondary Antibody: Goat anti-rabbit PE conjugated.

Western Blot of Rabbit Anti-TLR5 antibody. Lane A: Ramos. Lane B: RAW. Lane C: mouse spleen. Lane D: mouse lung. Lane E: rat lung lysate. Primary antibody: TLR5 at 2 µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for TLR5. Other band(s): none.

Immunohistochemistry of Rabbit Anti-TLR5 antibody. Tissue: stomach tumor. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: TLR5 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: TLR5 is a single-pass type 1 membrane protein. Staining: TLR5 as a precipitated red signal with hematoxylin purple nuclear counterstain.