Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.coli (BL21).
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Escherichia coli
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:1000-1:2000, WB: 1:500-1:1000
Application Notes:
Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and has been tested by 2D-Page, western blotting, and ELISA.
ELISA of Rabbit Anti-E.coli Host Cell Protein Antibody. Antigen: Total HCP protein. Coating amount: 0.1 µg per well. Primary antibody: E.coli HCP antibody at 10 µg/mL. Dilution series: 2-fold. Mid-point concentration: 5 ng/mL HCP antibody. Secondary antibody: Peroxidase rabbit secondary antibody at 1:20,000. Substrate: TMB (p/n TMBE-0100).
2D Western Blot of anti-E.coli Host Cell Protein antibody. Load: 35 µg Total HCP. Primary antibody: Rabbit anti-HCP antibody at 1:200 for overnight at 4C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 for 1 hour at RT.
2D SDS-PAGE: Load/Strip: 100µg E. coli HCP total lysate/strip. 2D Oriole stained gels.Western Blot of Anti-E.coli Total Host Cell Protein antibody:Primary antibody: Rabbit Anti-E.coli Total Host Cell Protein at 1:500 overnight at 2-8C.Secondary antibody: Goat anti-Rabbit IgG Cy5 Conjugated (p/n 611-110-122) at RT for 2hrs.Block: Blocking Buffer (p/n MB-070) for 1 hour at RT.
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