Histone H3 K4me1 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Histone H3 K4me1 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-401-P02
• Supplier Catalog Number: 200-401-P02
• Alternative Catalog Number: ROC-200-401-P02
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: DOT, ELISA, WB
• Species Reactivity: Human
• Immunogen: Histone H3 K4me1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Anti-Histone H3 Monomethyl Lys4 antibody, rabbit anti-Histone H3 K4 me1 antibody, H3 K4me1, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K4me1

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 126961
• UniProt: Q71DI3
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:3,000 - 1:10,000, ChIP: 2-5µg/million cells, IHC: 2 - 5 µg/ml, IF Microscopy: 2 - 5 µg/ml, IP: 2 mg/ml - 5 µg/ml, WB: 1:500 - 1:2,000
• Application Notes: Anti-Histone H3 [Monomethyl Lys4] antibody has been tested in ELISA, Dot blot, and Western Blot. Histone3 K4me1 is useful for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reac

Images

Western Blot of Rabbit anti-Histone H3 K4Me1 antibody. Lane 1: Raji Whole Cell Lysate (p/n W09-001-368). Load: 35 µg per lane. Primary antibody: H3K4me1 antibody at 1:2000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15kDa for Histone H3.