Anti-AGAP4 antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant His-tagged AGAP4 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-AGAP4 antibody, AGAP-4, AGAP 4, CTGLF1, MRIP2, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 4, Centaurin-gamma-like family member 1
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000-1:20,000, WB: 1:1000
Application Notes:
Anti-AGAP4 antibody has been tested for use in western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 73kDa in size corresponding to AGAP4 by western blotting in the appropriate cell lysate or ex
Western Blot of Rabbit Anti-AGAP4 Antibody. Lane 1: HeLa cells treated with HA/AGAP4. Lane 2: HeLa cells control. Lane 3: HeLa cells treated with AGAP4/siRNA. Load: 10 µg per lane. Primary antibody: AGAP4 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 71 kDa, 71 kDa for AGAP4. Other band(s): 42kDa Actin loading control.
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