Anti-Bid antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 14 amino acids near the internal region of human Bid.
Conjugation:
Unconjugated
Alternative Names:
Bid Antibody, FP497, BH3-interacting domain death agonist, p22 BID, BID
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5000 - 1:20,000, WB: 0.5 - 2 µg/mL
Application Notes:
Anti-Bid Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 22 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of Bid antibody in A549 cell lysates. Lane A: Bid antibody at 0.5 µg/mL. Lane B: Bid antibody at 1 µg/mL. Lane B: Bid antibody at 2 µg/mL. Load: 35 µg per lane. Primary antibody: Bid antibody at designated concentrations for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 22 kDa, 27 kDa for Bid. Other band(s): Bid splice variants and isoforms.
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