0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Swine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:1,000 - 1:5,000, WB: 1:250 - 1:1,000
Application Notes:
Anti-Carboxypeptidase B peroxidase Antibody has been tested in western blot and suitable to be assayed against 1.0 ug of Carboxypeptidase B [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])
Western Blot of Rabbit anti-Carboxypeptidase B Antibody Peroxidase Conjugated. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carboxypeptidase B Antibody Peroxidase Conjugated at 1:1,000 overnight at 4C. Secondary antibody: n/a. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 47 kDa, observed at 35 kDa for Carboxypeptidase B. Expected molecular weight for processed protein is ~34.7 kDa.
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