0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Swine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Application Notes:
Anti-CARBOXYPEPTIDASE B Biotin Antibody has been tested by Western Blot and is suitable to be assayed against 1.0 ug of Carboxypeptidase B in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbent
Western Blot of Rabbit anti-Carboxypeptidase B antibody Biotin conjugated. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carboxypeptidase B antibody Biotin conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP conjugated rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking buffer for Fluorescent Western Blotting (MB-070) at RT for 30 min. Predicted/Observed size: ~35 kDa
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