0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2,000-1:20,000
Application Notes:
Biotin Conjugated Anti-Streptavidin Antibody has been tested by dot blot and is suitable to be assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the recon
Western Blot of Rabbit Anti-Trypsin Inhibitor Antibody and Rabbit Anti-Streptavidin Antibody. Lane 1: Trypsin Inhibitor reduced. Lane 2: Trypsin Inhibitor non-reduced. Lane 3: Streptavidin reduced. Lane 4: Streptavidin non-reduced. Load: ~1ug per lane. Primary antibody: Primary antibody at 1:1000 for overnight at 4C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 18.8 kDa, ~15 kDa for Streptavidin, 24 kDa, ~20 kDa for Trypsin Inhibitor. Other band(s): none.
Dot Blot of Rabbit anti-Streptavidin Biotin Conjugated. Antigen: Lane 1 - Biotin conjugated Streptavidin Antibody. Lane 2 - Streptavidin Antibody. Load: 3-fold serial dilution starting at 200 ng. Secondary antibody: HRP Streptavidin at 1:40,000 for 60 min at RT. Block: 1% BSA-TTBS 30 min at RT.
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