Protein G Antibody Biotin Conjugated, Rabbit, Polyclonal
Biozol Catalog Number:
ROC-200-406-138
Supplier Catalog Number:
200-406-138
Alternative Catalog Number:
ROC-200-406-138
Manufacturer:
Rockland Immunochemicals
Host:
Rabbit
Category:
Antikörper
Application:
DOT
Immunogen:
Protein G [Streptococcus species]
Conjugation:
Biotin
Alternative Names:
rabbit anti-Protein G Antibody biotin conjugation, Rabbit anti Protein G biotin, Rabbit anti PG biotin
Clonality:
Polyclonal
Concentration:
10.0 mg/mL by UV absorbance at 280 nm
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:90,000 - 1:350,000
Application Notes:
Anti-Protein G Biotin Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Protein G in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic a
Dot Blot Results of Rabbit Anti-Protein G Antibody Biotin Conjugated. Lane A: Protein G. Lane B: Protein G reduced with TCEP. (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Anti-Protein G Biotin Conjugated at 1µg/mL for 1 hr at RT. Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30 mins at RT. Block: BlockOut (p/n MB-073) for 30 min RT.
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