Protein G Antibody Biotin Conjugated, Rabbit, Polyclonal

Catalog Number: ROC-200-406-138S
Article Name: Protein G Antibody Biotin Conjugated, Rabbit, Polyclonal
Biozol Catalog Number: ROC-200-406-138S
Supplier Catalog Number: 200-406-138S
Alternative Catalog Number: ROC-200-406-138S
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: DOT
Immunogen: Protein G [Streptococcus species]
Conjugation: Biotin
Alternative Names: rabbit anti-Protein G Antibody biotin Conjugation, biotin conjugated rabbit anti-Protein G Antibody, Protein G BAC
Clonality: Polyclonal
Concentration: 1.0 mg/ml by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:9,000 - 1:35,000
Application Notes: Anti-Protein G Biotin Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Protein G in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic a
Dot Blot Results of Rabbit Anti-Protein G Antibody Biotin Conjugated. Lane A: Protein G. Lane B: Protein G reduced with TCEP. (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Anti-Protein G Biotin Conjugated at 1µg/mL for 1 hr at RT. Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30 mins at RT. Block: BlockOut (p/n MB-073) for 30 min RT.