Anti-Trypsin Biotin Antibody has been tested by ELISA and western blot. Researchers should determine optimal titers for applications that are not stated below.
Western Blot of Rabbit anti-Trypsin Antibody Biotin Conjugated. Lane 1: Trypsin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Trypsin Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 26 kDa, 26 kDa for Trypsin. Other band(s): autocatalytic fragment of Trypsin, ~10 kDa.
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