Adenosine Deaminase Antibody Biotin Conjugated, Rabbit, Polyclonal Preis auf Anfrage

Product Overview

• Article Name: Adenosine Deaminase Antibody Biotin Conjugated, Rabbit, Polyclonal Preis auf Anfrage
• Biozol Catalog Number: ROC-200-406-140S
• Supplier Catalog Number: 200-406-140S
• Alternative Catalog Number: ROC-200-406-140S
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Bovine
• Immunogen: Adenosine Deaminase [Calf Spleen]
• Conjugation: Biotin
• Alternative Names: rabbit anti-Adenosine Deaminase Antibody biotin Conjugation, biotin conjugated rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 280712
• UniProt: P56658
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Bovine
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Anti-Adenosine Deaminase Biotin Antibody has been tested by western blot. This product is suitable to be assayed against 1.0 ug of Adenosine Deaminase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-

Images

Rockland biotin conjugated anti adenosine deaminase (200-406-140) was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA. Blot was incubated with 200-406-140 lot 5628 (1:5K in TBS, ON 4C), washed 3X in TBS and incubated for 30 minutes with Dylight 488 conjugated Streptavidin (S000-41 lot 20833 1:5K in MB-070). Blot was imaged with the Biorad VersaDoc imaging system.