0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: User Optimized, WB: User Optimized
Application Notes:
Anti-Alkaline Phosphatase Biotin Antibody has been tested by ELISA and dot blot. This product is assayed against 1.0 ug of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethy
Dot Blot for Anti-ALKALINE PHOSPHATASE (Human Intestine) (RABBIT) Antibody Biotin Conjugated. Lane 1: 100 ng Alkaline Phosphatase. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: Anti-Alkaline Phosphatase Antibody Biotin Conjugated at 10 µg/ml at RT for 1 hour. Secondary Antibody: Streptavidin Peroxidase Conjugated (p/n S000-03) at a 1:40,000 dilution at RT for 30 minutes. Block: MB-070 at RT for 30 minutes.
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