0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Bovine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:250,000, IHC: User Optimized, WB: 1:500 - 1:3,000
Application Notes:
Anti-Carboxypeptidase A has been tested by western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit Anti-Carboxypeptidase A primary antibody. Lane 1: Carboxypeptidase A. Load: 50 ng per lane. Primary antibody: Carboxypeptidase A primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Blocking: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 34 kDa, 34 kDa for Carboxypeptidase A. Other band(s): Carboxypeptidase A splice variants and isoforms.
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