Lipoamide Dehydrogenase Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Lipoamide Dehydrogenase Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-4160-0100
• Supplier Catalog Number: 200-4160-0100
• Alternative Catalog Number: ROC-200-4160-0100
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Porcine
• Immunogen: Lipoamide Dehydrogenase [Porcine Heart]
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Lipoamide Dehydrogenase Antibody, Dihydrolipoyl dehydrogenase mitochondrial, Dihydrolipoamide dehydrogenase, Lipoamide Dehydrogenase

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 397129
• UniProt: P09623
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Swine
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Anti-Lipoamide Dehydrogenase has been tested by ELISA and western blot and is assayed against 1.0 ug of Lipoamide Dehydrogenase [Porcine Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code 611-1302

Images

Western Blot of Rabbit Anti-Lipoamide Dehydrogenase Antibody. Lane 1: Lipoamide Dehydrogenase Reduced [0.05µg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB210-0500). Lane 3: Lipoamide Dehydrogenase Non-Reduced [0.05µg]. Primary Antibody: Anti-Lipoamide Dehydrogenase (p/n 200-4160) at 1:1000 overnight at 2-8C. Secondary Antibody: Goat anti-Rabbit IgG [H&L] HRP (p/n 611-1302) at 1:40,000 for 30mins at RT. Blocking: BlockOut buffer (p/n MB-073) for 1hr at RT. Expected MW: 55kDa. Exp: 10 secs.