Anti-Lipoamide Dehydrogenase has been tested by ELISA and western blot and is assayed against 1.0 ug of Lipoamide Dehydrogenase [Porcine Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code 611-1302
Western Blot of Rabbit Anti-Lipoamide Dehydrogenase Antibody. Lane 1: Lipoamide Dehydrogenase Reduced [0.05µg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB210-0500). Lane 3: Lipoamide Dehydrogenase Non-Reduced [0.05µg]. Primary Antibody: Anti-Lipoamide Dehydrogenase (p/n 200-4160) at 1:1000 overnight at 2-8C. Secondary Antibody: Goat anti-Rabbit IgG [H&L] HRP (p/n 611-1302) at 1:40,000 for 30mins at RT. Blocking: BlockOut buffer (p/n MB-073) for 1hr at RT. Expected MW: 55kDa. Exp: 10 secs.
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