Anti-Phospho Enol Pyruvate Carboxylase antibody has been tested by western blotting and ELISA is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.
Western Blot of Rabbit Anti-Phospho Enol Pyruvate (PEP) Carboxylase antibody. Lane 1: Phospho Enol Pyruvate (PEP) Carboxylase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Phospho Enol Pyruvate (PEP) Carboxylase primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 100 kDa, 100 kDa for Phospho Enol Pyruvate (PEP) Carboxylase. Other band(s): None.
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