Urease Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Urease Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-4182-0100
• Supplier Catalog Number: 200-4182-0100
• Alternative Catalog Number: ROC-200-4182-0100
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Plant
• Immunogen: Urease [Jack Bean]
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Urease Antibody, Urea Amidohydrolase antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 83831
• UniProt: P07374
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Jack Bean
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Anti-Urease Antibody has been tested in western blot and suitable against 1.0 ug of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code 611-1302 and (ABTS (2,2-azino-bis-[3-ethylbenthia

Images

Western Blot of Rabbit anti-Urease (Jack Bean) Antibody. Lane 1: Urease (Jack Bean). Load: 50 ng per lane. Primary antibody: Urease primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 90 kDa, 90 kDa for Urease.