0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Escherichia coli
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
Application Notes:
Anti-Alkaline phosphatase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid
Western Blot of Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody Peroxidase Conjugated. Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody Peroxidase Conjugated at 1:1,000 overnight at 4C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).
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