0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Bovine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2,000 - 1:10,000, WB: 1:500- 1:2,500
Application Notes:
Anti-Catalase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Catalase [Bovine Liver] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code
Western Blot of Rabbit anti-Catalase antibody. Lane R: Reduced samples of purified Catalase. Lane NR: Non-reduced samples of purified Catalase. Load: ~1ug of protein per lane. Primary antibody: Catalase antibody at 1:1000 for overnight at 4C. Secondary antibody: Dylight488(TM) rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 59.9 kDa, 55 kDa for catalase. Other band(s): none.
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