Anti-Asparaginase Antibody was produced by repeated immunizations with E. coli Asparaginase protein.
Conjugation:
HRP
Alternative Names:
rabbit anti-L-Asparaginase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Escherichia coli
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:1,000 - 1:5,000, WB: 1:200 - 1:1,000
Application Notes:
Anti-Asparaginase Peroxidase conjugated antibody has been tested by western blotting to detect a single band of the expected apparent molecular weight and by ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot of Peroxidase conjugated Rabbit anti-L-ASPARAGINASE antibody. Lane 1: L-ASPARAGINASE. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 30 kDa for L-ASPARAGINASE. Other band(s): L-ASPARAGINASE splice variants and isoforms.
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