0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Lima Bean
Antibody Type:
Primary Antibody
Application Dilute:
IP: 1:100, WB: 1:250
Application Notes:
Anti-Trypsin Inhibitor Peroxidase Conjugated Antibody has been tested by Dot blot and is suitable to be assayed against 1.0 ug of Trypsin Inhibitor [Lima Bean] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])
Dot Blot Results of Rabbit Anti-Trypsin Inhibitor Antibody Peroxidase Conjugated. Trypsin Inhibitor (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Anti-Trypsin Inhibitor HRP at 1.0µg/mL for 1hr at RT. Secondary Antibody: none. Blocking Buffer: BlockOut Buffer (p/n MB-073) for 30mins at RT. Exposure: 0.25sec.
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