0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Bovine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:20,000, WB: 1:500- 1:2,000
Application Notes:
Anti-Superoxide Dismutase Peroxidase Conjugated Antibody has been tested by Western blot and is suitable to be assayed against 1.0 ug of Superoxide Dismutase [Bovine erythrocytes] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoli
Western Blot of HRP Conjugated Superoxide Dismutase Antibody. Lane 1: Superoxide Dismutase Load: 50 ng per lane. Primary antibody: HRP Conjugated Superoxide Dismutase Antibody at 1:1,000 overnight at 4C. Secondary antibody: none Detected directly using FEMTOMAX Substrate (p/n FEMTOMAX-110A and FEMTOMAX-110B). Block: MB-070 for 30 min at RT. Predicted/Observed size: 15.6 kDa, 15.6 kDa.
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