Ovalbumin Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Ovalbumin Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-4633-0100
• Supplier Catalog Number: 200-4633-0100
• Alternative Catalog Number: ROC-200-4633-0100
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: DOT, ELISA, WB
• Species Reactivity: Gallus
• Immunogen: Ovalbumin [Hen Egg White]
• Conjugation: Biotin
• Alternative Names: rabbit anti-Ovalbumin Antibody biotin Conjugation, biotin conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 396058
• UniProt: P01012
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:500 - 1:2,500
• Application Notes: Anti-Ovalbumin Antibody Biotin Conjugated is tested by ELISA, dot blot, and western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications th

Images

Western Blot of Rabbit anti-Ovalbumin antibody. Lane 1: ~1 µg Ovalbumin protein reduced. Lane 2: 0.25 µg Ovalbumin protein reduced. Lane 3: ~1 µg Ovalbumin protein non-reduced. Lane 4: 0.25 µg Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block: MB-070 buffer overnight at 4C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin. Other band(s): none.

Western blot of Biotin conjugated Rabbit Anti-Ovalbumin primary antibody. Lane 1: Ovalbumin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ovalbumin biotin conjugated primary antibody at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.