0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Escherichia coli
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
Application Notes:
Anti-Alkaline Phosphatase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-az
Western Blot of Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Streptavidin (S000-03) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).
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